Description
This study investigates the influence of Coriolis force on transport and hybridization of DNA molecules in CD microfluidics platform where centrifugal force is used as the driving force. While the effect of Coriolis force on fluid flow in CD microfluidics channels has been studied experimentally and numerically only recently, its influence on DNA molecule migration and hybridization has not been investigated so far. This study addresses this phenomenon through numerical simulation and demonstrates that for most practical geometrical configurations and angular velocity ranges reported in the literature, the Coriolis force introduces significant qualitative and quantitative variations in the hybridization of DNA molecules, particularly at locations near the periphery. Further, to validate the numerical approach developed here, this research carried out several experiments on microfluidics channels in a CD platform. Both numerically and experimentally, Coriolis effect is observed to be significantly influenced by channel width and angular rotations. Our results indicate that for low viscosity fluids, angular velocities as low as 25 rad/sec could introduce Coriolis force that is as high as at least 25% of the main driving centrifugal force.
