This research investigates 3-Dimensional pumping in a compact disc (CD) microfluidics platform involving two or more layers. The traditional 2-Dimensional pumping in CD microfluidics platform has a few restrictions such as reduced hybridization efficiency and insufficient species collection which 3-Dimensional pumping can achieve. This research focuses on a 3-Dimensional pumping, pumps fluid from the center of the CD on one platform to a CD that is placed on another platform using a valveless vertical channel. Fluid is pumped using a continuous flow from reservoir into channels mounted on a CD with varying angular velocity. With this architecture many fluidic functions could be repeated and much more efficient results could be generated. The main outcomes of this research involve designing, analyzing and microfabricating a 3-Dimensional compact disc platform. The research has demonstrated 3-Dimensional pumping under centrifugal force where a fully developed fluid flow is observed in multitude of layers with a velocity of 10-15meter/second for channels 7mm length, 2mm depth and 0.5mm width are demonstrated.