Biomimetic microdevices offer a viable, laboratory-based system capable of reproducing complex organ-level structures. These systems not only offer the possibility of altering numerous parameters but also the ability of monitoring their effects in real time, effectively making them an ideal drug testing tool. However, while several such systems exist or are currently under development, they still require extensive skills and labor as they rely on additional secondary assays for detection. In this research, we replicate the Lung-on-a- Chip platform with a cystic fibrosis cell line, mimicking the alveolar-capillary interface, then extend the functionality of the chip by integrating an oxygen optode, an optical biosensing unit which has been specifically conjugated to respond to oxygen. The oxygen concentrations within the chip can be monitored and the resulting data used to build a model for the progression of the disease.