In this research, the electrical and electrochemical characterization of geometrical and surface treatment effects on the behavior of Glassy Carbon (GC) microelectrodes have been studied. we report on the electrochemical tunability of patternable Glassy Carbon microelectrode probe structures for potential applications in bio-electrical signal recording and stimulation. The structures, made from lithographically patterned negative photoresist and subsequently pyrolyzed, have excellent electrochemical properties and electrochemical stability in biological fluids. Driven by the need of decreasing the damages and risks associated with neuroprosthetic implants, the present work focuses the electrochemical properties of glassy carbon such as Impedance and charge density before and after plasma etching as well as in different structures of glassy carbon electrodes including pillars and disks. Analyzing these parameters helps study the changes in the impedance as well as the amount of charge delivered by the material in a biological solution simulating the extracellular fluid. Another important contribution of this study is the investigation of the stability of the electrochemical properties of the material with change in the values of the parameters of the equivalent circuit for Electrochemical Impedance Spectroscopy. This research aims to depict the superiority of glassy carbon for neuroprosthetic devices, replacing current materials widely used in the fabrication of neural probes.