Background: Fear of crime in the neighborhood is hypothesized to play role in whether individuals will participate in physical activity. Research on this subject has shown inconsistent results. Studies have found three different outcomes, no significant association, a positive association, and a negative association between physical activity and fear of crime. The majority of studies have shown that those who do not fear crime in their neighborhood are more likely to be physically active then those who do. Purpose: This study was conducted to examine the relation of fear of crime within the neighborhood and physical activity level in middle-aged adults. Methods: Participants (n=680) completed three different sets of surveys and measurements that included a self-administered survey, telephone survey, and a home visit where physiological measures were taken. Recruitment took place from 2007 to 2012 in Maricopa County, Arizona. Multinomial logistic regression was used to analyze the data from a cross sectional study of adults, aged 40-65 years. Results: The analysis shows a significant association between fear of crime and physical activity level when stratified by race and controlling for gender, age, BMI, education, and general health. Discussion: These findings indicate that the physical activity level of an adult is affected by their fear of crime in the neighborhoods where they live.