Extracellular vesicles (EVs) are classically known as a form of communication between cells, but more recently researchers have to started to examine the potential of EVs as biomarkers for disease and even as a potential means of transporting therapeutic cargo. A previous method used to isolate EVs from conditioned cell culture media was cushioned- density gradient ultracentrifugation paired with size exclusion chromatography. I propose that immunomagnetic separation is a more efficient and biologically selective method for extracting EVs and characterizing their molecular cargo. I compared the efficiency of two immunomagnetic separation beads from two different biotechnology companies to extract EVs expressing one of three tetraspanin proteins – CD9, CD63, CD81. I used vesicle flow cytometry and immunoblotting to determine the efficiency of these two different systems to extract the desired EVs. I used RNAseq to examine the molecular cargo composition of these tetraspanin expressing EVs. Once the workflow was optimized on HEK293 and DiFi cell supernatant, I used this optimized protocol to extract EVs and characterize their molecular cargo from three placenta-derived in vitro systems. I successfully developed an immunomagnetic separation-based workflow to extract EVs from conditioned cell culture media. I used this optimized workflow to examine extracellular vesicles and their molecular cargo extracted from three placenta-derived in vitro systems. This workflow efficiently extracted EVs from the placenta condition cell culture medias, which suggests a potential for broad range effectiveness of this workflow. Finally, I compared the data for these three cell lines to determine their potential as an in vitro system used to accurately represent the placenta. Placental explants are the closest in vitro system to the in vivo placenta, but they are not sustainable in cell culture long term. Comparing the data from BeWo cells and trophoblast stem cells (TSCs) to the placental explants, I concluded that TSCs are a better candidate as an in vitro system for future placental dysfunction studies.