Phage vary in structure and function and occupy diverse areas of the biosphere, such as the ocean, soil, and within animals. Our knowledge of the interactions between bacteriophage and humans is limited, and few studies examine the interactions between phage and eukaryotic cells. Phage are being evaluated as a phage therapy treatment for infected burn wounds in Europe. Oral administration of phage to human patients and mice revealed the presence of said phage within the bloodstream and urinary tract hours later. These studies demonstrate that the body is accessible to phage, yet the mechanism by which phage are able to access these areas of the body remains unknown. We propose phage transcytosis across epithelial cell layers as the mechanism that allow phage to permeate the body. For these assays Transwells® are used. Transwells® tissue culture wells that are widely used in transcytosis assays for studying transportation and drug transport across cell layers. Transwells are composed of a porous membrane on which eukaryotic cells were seeded and grown to form a confluent cell monolayer. Phage were then applied to the cells to test phage passage across that cell layer. From these experiments, evidence is provided of phage transcytosis across cells from the kidney and the gut in a unidirectional motion from the apical to basal direction. Phage were visualized within cells via confocal microscopy and evidence that they are transported across the epithelial cell layer via vesicle-mediated transcytosis are included here. The results from these experiments will provide greater understanding of the roles that phage play in the human body.